Introducing Kronos Dio operation workflow, including PC software operation as well as experimental protocols.
Apoptosis analysis by
AB3000 Cellgraph and AB-2550 KronosDio
AB-2550 Kronos Dio
Kronos Dio(AB-2550) is a luminometer for detecting bioluminescence in living cells and tissues by PMT. It is available to control temperature and CO2 gas concentration inside Kronos Dio such as an incubator for cell & tissue culturing. Besides Kronos Dio gives superior performance for multi-color assay, which can detect different colors of luminescence up to three in the same time by an internal optical filtering system. Kronos Dio is performed to detect high sensitivity for dim and weak luminescence consecutively in culture samples such as cells and tissues in 35mm culture dishes for a long time. Therefore, it is not required to prepare several samples for measuring each time point to chase alteration in chronologic bioluminescence. In this way, it is very reasonable and convenient against conventional ways and methods in terms of saving time, accuracy, and efficiency.
*GPC laboratory CO. LTD. provides a useful cell line carrying luciferase as a reporter gene for assessing circadian rhythm by Bmal1 gene expression.